Serviço de Biologia Molecular preço
Subcloning cDNA/DNA PCR cloning (classic)
vectors available with GFP, mCherry, RFP, V5, myc, FLAG, SNAP, GST, HIS, MBP tags
DNA Cloning (≤ 500 bp) 150-300€
DNA Cloning (501 bp -1.5 kb) 200-500€
DNA Cloning (≥1.5 kb) 40€/h
Subcloning (classic)
vectors available with GFP, mCherry, RFP, V5, myc, FLAG, SNAP, GST, HIS, MBP tags
DNA Subcloning (≤2,000 bp) 200-300€
DNA Subcloning (>2,000 bp) 40€/h
Site-directed mutagenesis
Site-directed mutagenesis (1-2 reactions/construct) 300-400€
Site-directed mutagenesis (3-5 reactions/construct) 400-500€
cDNA/DNA PCR cloning (Gateway®)
DNA PCR cloning into pENTR (≤2,000 bp) (eg pCR8) 200-300€
DNA PCR cloning into pENTR (>2,000 bp) 40€/h
Subcloning into prokaryotic or mammalian vectors, several tags available
Subcloning into lentiviral vectors (vectors available pLenti6, pLenti6/CMV) 200-300€
Subcloning into adenoviral vectors (vectors available pAd, pAd/CMV) 300-500€
Virus production
Lentivirus production 300-400€
Adenovirus production 200-500€
Protein production (prokaryotic system) 500-1000€
Protein production (eukaryotic system) 40€/h
Other services available
(all reagents/materials and consumables are included)