Microfluidic Device Intergrading a Selectively Permeable Membrane and a 3D Vascular Network for the In Vitro Development of Anatomical-functional Barrier

Three-Dimensional Model of Early Diabetic Retinopathy

• 2022-2025 - “NRF2 as a novel therapeutic target in early and intermediate age-related macular degeneration-NASCENT” 
  HR22-00569 ”la Caixa” Foundation; PL: Miguel Seabra; PI: Antonio Cuadrao, UAM, ES
  SABER MAIS
• 2022-2024 - "Utilização de um modelo de organoide de retina humano de Retinopatia diabética para identificação de novos fármacos”
  Bolsa Charneco da Costa - Sociedade Portuguesa de Diabetologia
  PI: Sandra Tenreiro
• 2021-2022 - "Mimetizando as condições de hiperglicemia em organoides de retina humanos: um modelo 3D de Retinopatia diabética precoce para identificação de novos fármacos”
  iNOVA4Health.
  PI: Sandra Tenreiro; Co-PIs: Gabriela Silva (CEDOC) and Cláudia Nunes dos Santos (CEDOC).
• 2021-2022 - "Exossomas na proteostase transcelular em Degenerescência Macular relacionada com a idade"
  iNOVA4Health.
  Co-PI: Sandra Tenreiro;PI: João Ferreira (CEDOC).
• 2018-2022- "Recuperação da função neuronal e promoção de neuroproteção em retinopatia diabética”
  PTDC/MED-PAT/29656/2017 finaciado pela Fundação para a Ciência e Tecnologia
  PI: Sandra Tenreiro

• Modelling neurodegeneration and inflammation in early diabetic retinopathy using 3D human retinal organoids
• Age-Related Changes of the Synucleins Profile in the Mouse Retina
• Retinal Progression Biomarkers of Early and Intermediate Age-Related Macular Degeneration. Life. 2021;
• A biophysical perspective on the unexplored mechanisms driving Parkinson's disease by amphetamine-like stimulants. 2021;
• Neuroprotection or Neurotoxicity of Illicit Drugs on Parkinson's Disease. Life. 2020;
• The synthetic cannabinoid JWH-018 modulates S. cerevisiae energetic metabolism. FEMS Yeast Research. 2019 .

• Rojo AI, Buttari B, Cadenas S, Carlos AR, Cuadrado A, Falcão AS, López MG, Georgiev MI, Grochot-Przeczek A, Gumeni S, Jimenez-Villegas J, Horbanczuk JO, Konu O, Lastres-Becker I, Levonen AL, Maksimova V, Michaeloudes C, Mihaylova LV, Mickael ME, Milisav I, Miova B, Rada P, Santos M, Seabra MC, Strac DS, Tenreiro S, Trougakos IP, Dinkova-Kostova AT. Model organisms for investigating the functional involvement of NRF2 in non-communicable diseases. Redox Biol. 2024 Dec 16;79:103464. doi: 10.1016/j.redox.2024.103464.
• Teixeira J, Lumack do Monte Z, Tenreiro S, Salema-Oom M, Silva DC, Saramago B, Paula Serro A. Citicoline eluting hydrogels for therapeutic contact lenses intended to treat neurodegenerative diabetic ocular diseases. Int J Pharm. 2024 Dec 25;667(Pt A):124908. doi: 10.1016/j.ijpharm.2024.124908.
• Fonseca AF, Coelho R, da-Silva ML, Lemos L, Hall MJ, Oliveira D, Falcão AS, Tenreiro S, Seabra MC, Antas P. Modeling Choroideremia Disease with Isogenic Induced Pluripotent Stem Cells. Stem Cells Dev. 2024 Oct;33(19-20):528-539. doi: 10.1089/scd.2024.0105. Epub 2024 Aug 16.
• de Lemos, L., Antas, P., Ferreira, I.S. et al. Modelling neurodegeneration and inflammation in early diabetic retinopathy using 3D human retinal organoids. In vitro models 3, 33–48 (2024). https://doi.org/10.1007/s44164-024-00068-1
• Flores R, Fradinho AC, Pereira RS, Mendes JM, Seabra MC, Tenreiro S, Carneiro Â. Identifying Imaging Predictors of Intermediate Age-Related Macular Degeneration Progression. Transl Vis Sci Technol. 2023 Jul 3;12(7):22. doi: 10.1167/tvst.12.7.22.
• Dias SB, de Lemos L, Sousa L, Bitoque DB, Silva GA, Seabra MC, Tenreiro S. 2023. Biomolecules. Age-Related Changes of the Synucleins Profile in the Mouse Retina. 2023 Jan 15; 13(1):180. doi: 10.3390/biom13010180.
• Cabral D, Ramtohul P, Kaden TR, Tenreiro S, Seabra MC, Freund KB.Eye (Lond). 2022. Hyperpigmented epiretinal membrane in macular telangiectasia type 2: imaging characteristics and correlation with transretinal pigment migration.2022 Sep 22. doi: 10.1038/s41433-022-02260-7. Online ahead of print.
• Cabral D, Fradinho AC, Pereira T, Ramakrishnan MS, Bacci T, An D, Tenreiro S, Seabra MC, Balaratnasingam C, Freund KB.Transl Vis Sci Technol. 2022. Macular Vascular Imaging and Connectivity Analysis Using High-Resolution Optical Coherence Tomography. 2022 Jun 1;11(6):2. doi: 10.1167/tvst.11.6.2.
• Flores R, Carneiro Â, Neri G, Fradinho AC, Quenderra B, Barata MJ, Tenreiro S, Seabra MC. Diagnostics (Basel). 2022. Choroidal Vascular Impairment in Intermediate Age-Related Macular Degeneration. 2022 May 22;12(5):1290. doi: 10.3390/diagnostics12051290.
• Camacho P, Ribeiro E, Pereira B, Varandas T, Nascimento J, Henriques J, Dutra-Medeiros M, Delgadinho M, Oliveira K, Silva C, Brito M. DNA methyltransferase expression (DNMT1, DNMT3a and DNMT3b) as a potential biomarker for anti-VEGF diabetic macular edema response. Eur J Ophthalmol. 2023 Apr 20:11206721231171623. doi: 10.1177/11206721231171623. Epub ahead of print. PMID: 37082811.
• Menino J, Camacho P, Coelho A. Initial medication adherence in newly diagnosed glaucoma patients: three adherence measures. Int J Ophthalmol. 2023 Apr 18;16(4):630-637. doi: 10.18240/ijo.2023.04.18. PMID: 37077479; PMCID: PMC10089901.
• Flores R, Carneiro A, TENREIRO S, Seabra MC. 2021. Retinal Progression Biomarkers of Early and Intermediate Age-Related Macular Degeneration. Life. Doi: 10.3390/life12010036. Online ahead of print. Review.
• Flores R, Carneiro A, Vieira M, TENREIRO S, Seabra MC. 2021. Age-Related Macular Degeneration: Pathophysiology, Management, and Future Perspectives. Ophthalmologica. Doi: 10.1159/000517520. Online ahead of print. Review.
• Escrevente C, Falcão AS, Hall MJ, Lopes-da-Silva M, Antas P, Mesquita MM, Ferreira IS, Cardoso MH, Oliveira D, Fradinho AC, Ciossek T, Nicklin P, Futter CE, TENREIRO S, Seabra MC. 2021. Formation of Lipofuscin-Like Autofluorescent Granules in the Retinal Pigment Epithelium Requires Lysosome Dysfunction. Investigative Ophthalmology and Visual Science. 62:39.
• Ferreira C, Couceiro J, TENREIRO S, Quintas A. 2021. A biophysical perspective on the unexplored mechanisms driving Parkinson's disease by amphetamine-like stimulants. Neural Regeneration Research. 16:2213-4. Review.
• Flores R, Carneiro A, Serra J, Gouveia N, Pereira T, Mendes JM, Coelho PS, TENREIRO S, Seabra MC. 2021. Correlation Study Between Drusen Morphology and Fundus Autofluorescence. Retina. 41:555–62.
• Klionsky DJ, et al, 2021 Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) Autophagy. 17:1-382.
• Ferreira C, Almeida C, TENREIRO S, Quintas A. 2020. Neuroprotection or Neurotoxicity of Illicit Drugs on Parkinson's Disease. Life 10:86. doi: 10.3390/life10060086. Review.
• Ferreira C, Couceiro J, Família C, Jardim C, Antas P, Santos CN, Outeiro TF, TENREIRO S, Quintas A. The synthetic cannabinoid JWH-018 modulates S. cerevisiae energetic metabolism. 2019. FEMS Yeast Research. 19 :foz042. DOI: 10.1093/femsyr/foz042

• Melhor apresentação oral na Categoria Investigação Básica do iMED Innovate Competition 2023, para Olga Carreira.
• Melhor Poster iMED 2022 para Marta Cação
  SABER MAIS
• Bolsa Charneco da Costa atribuída pela Sociedade Portuguesa de Diabetologia
• O artigo Ferreira et al., 2020 foi destacado pelo Editor Prof. Dr. Ramón Cacabelos da revista Life como o melhor artigo 2020.
• O artigo Ferreira et al., 2019 foi destacado pelo Editor da revista FEMS Yeast Research como o melhor artigo 2019 e esteve no top 10 dos papers mais pontuados desse ano.

• Agnieszka Rybak-Wolf, Berlin Institute for Medical Systems Biology of the Max Delbrück Center
• Alexandre Quintas, Instituto Superior de Ciências da Saúde Egas Moniz, Lisboa
• António Cuadrado, Faculty of Medicine Autonomous University of Madrid, Spain
• António Jacinto, NOVA Medical School, UNL
• Catarina Brito, ITQB NOVA - Instituto de Tecnologia Química e Biológica António Xavier, Oeiras.
• Cláudia Nunes dos Santos, NOVA Medical School, UNL
• Evguenia Bekman, Stem Cell Engineering and Regenerative Medicine Research Group, Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Lisboa.
• Federico Herrera, Faculty of Sciences, Universidade de Lisboa
• Gabriela Silva, NOVA Medical School, UNL
• Mireya Plass, Institut d'Investigació  Biomèdica de Bellvitge, IDIBELL
• Michael Robson, Berlin Institute for Medical Systems Biology of the Max Delbrück Center
• Nuno Matela, Instituto de Biofísica e Engenharia Biomédica, Departamento de Física da Faculdade de Ciências da Universidade de Lisboa
• Rune Matthiesen, NOVA Medical School, UNL
• Susana Simões and Lino Ferreira, Faculty of Medicine, University of Coimbra.
• Paulo Pereira, NOVA Medical School, UNL