Localização

NOVA Medical School | Universidade NOVA de Lisboa Rua Câmara Pestana 6, room 3.25 1150-199 Lisboa, Portugal

Paulo Gameiro

Investigador Investigador Principal

Desde cedo, desenvolvi uma profunda curiosidade sobre as vias metabólicas e evolução ao descobrir que as células utilizam pequenas moléculas para manter as suas funções vitais de forma extraordinariamente regulada e conservada. Isso levou-me a estudar bioquímica, tendo-me focado posteriormente no estudo do metabolismo central do carbono, na biologia do RNA e nos erros inatos do metabolismo. No nosso laboratório usamos abordagens bottom-up e holísticas, por forma a obter uma compreensão da fisiopatologia mitocondrial desde a sua base ao explorar, de forma integrada, os componentes metabólicos e suas interações.

ARTIGOS:

  • Barbieri L, Veliça P, Gameiro PA, Cunha PP, Foskolou IP, Rullman E, Bargiela D, Johnson RS, and Rundqvist, H. (2023). Lactate exposure shapes the metabolic and transcriptomic profile of CD8+ T cells. Frontiers in Immunology, 14.
  • Gameiro PA*, Encheva V, Dos Santos MS, MacRae JI, and Ule J. (2021). Metabolic turnover and dynamics of modified ribonucleosides by 13C labelling. Journal of Biological Chemistry 297(5):101294. | * Corresponding author.
  • Rundqvist H, Veliça P, Barbieri L, Gameiro PA, Bargiela D, Gojkovic M, Mijwel S, Reitzner SM, Wulliman D, Ahlstedt E, Ule J, Östman A, and Johnson RS. (2020). Cytotoxic T-cells mediate exercise-induced reductions in tumor growth. Elife 9.
  • Gameiro PA and Struhl K. (2018). Nutrient deprivation elicits a transcriptional and translational inflammatory response coupled to decreased protein synthesis. Cell Reports 24(6):1415-1424 | Commented in Trends Biochem Sci 43 (11), 849-852 ‘Translation Links Nutrient Availability with Inflammation’.
  • Li BB, Qian C, Gameiro PA, Liu C-C, Jiang T, Roberts TM, Struhl K, and Zhao JJ. (2018). Targeted profiling of RNA translation reveals mTOR-4EBP1/2-independent translation regulation of mRNAs encoding ribosomal proteins. Proc. Natl. Acad. Sci. U. S. A. 115, E9325–E9332.
  • Okazaki A, Gameiro PA, Christodoulou D, Laviollette L, Schneider M, Chaves F, Stemmer-Rachamimov A, Yazinski SA, Lee R, Stephanopoulos G, Zou L, and Iliopoulos O. (2017). Glutaminase and poly(ADP-ribose) polymerase inhibitors suppress pyrimidine synthesis and VHL-deficient renal cancers. Journal of Clinical Investigation. 127(5): 1631–1645. | Commented in Nat Rev Nephrol 13 (6), 320 ‘Kidney Cancer: Targeting Metabolism in RCC’
  • Gameiro PA, Yang J, Metelo AM, Pérez-Carro R, Baker R, Wang Z, Arreola A, Rathmell WK, Olumi A, López-Larrubia P, Stephanopoulos G, and Iliopoulos O. (2013). In Vivo HIF-Mediated Reductive Carboxylation Is Regulated by Citrate Levels and Sensitizes VHL-Deficient Cells to Glutamine Deprivation. Cell Metabolism 17(3):372-85. | Commented in Nat Rev Cancer 13 (5), 293 ‘Metabolism: Glutamine connections’
  • Nicolay BN, Gameiro PA, Tschöp K, Korenjak M, Heilmann AM, Asara JM, Stephanopoulos G, Iliopoulos O, and Dyson NJ. (2013). Loss of RBF1 changes glutamine catabolism. Genes and Development. 27, 182–196.
  • Gameiro PA, Laviolette LA, Kelleher JK, Iliopoulos O, and Stephanopoulos G. (2013). Cofactor balance by nicotinamide nucleotide transhydrogenase (NNT) coordinates reductive carboxylation and glucose  catabolism in the TCA cycle. Journal of Biological Chemistry 288(18):12967-77. | Paper of the week
  • Metallo CM, Gameiro PA, Bell EL, Mattaini KR, Yang J, Hiller K, Jewell CM, Johnson ZR, Irvine DJ, Guarente L, Kelleher JK, Vander Heiden MG, Iliopoulos O, and Stephanopoulos G. (2012). Reductive glutamine metabolism by IDH1 mediates lipogenesis under hypoxia. Nature 481 (7381):380-4.
  • Gaglio D, Metallo CM, Gameiro PA, Hiller K, Danna LS, Balestrieri C, Alberghina L, Stephanopoulos G, and Chiaradonna F. (2011). Oncogenic K-Ras decouples glucose and glutamine metabolism to support cancer cell growth. Molecular Systems Biology 7, 523.

CAPÍTULOS DE LIVROS:

  • Zhang J*, Ahn WS*, Gameiro PA*, Keibler MA, Zhang Z, and Stephanopoulos G. (2014). 13C isotope assisted methods for quantifying glutamine metabolism in cancer cells. Methods in Enzymology (542):269-89 (Chapter 19). | * Equal contribution
  • Gameiro PA, Metallo CM, and Stephanopoulos G. Systems-level analysis of cancer metabolism. Systems Metabolic Engineering pp 349–381 (Chapter 11). Springer (2012)

PREPRINTS:

  • Gameiro PA*, Foskolou IP*, Martens A, Kuret K, de los Mozos IR, Hozjan Ž, Kot V, Faraway R, Vermeulen M, Wolkers M, Johnson RS, Ule J. (2024). Meta-unstable mRNAs in activated CD8+ T cells are defined by interlinked AU-rich elements and m6A mRNA methylation. | * equal contribution. doi.org/10.21203/rs.3.rs-4768725/v1. Under Review

PATENTES:

  • (2018) Metabolic Gene, Enzyme, and Flux Targets for Cancer Therapy (US Patent No. US10064885B2). 
    Inventors: Stephanopoulos G, Metallo CM, Kelleher JK, Iliopoulos O, and Gameiro PA.

 

Financiamento:
2022 | “Junior Leader – Incoming” Fellowship, ref: 116925, “la Caixa” Foundation
2022 | Scientific Employment Stimulus, ref: 2021.01737.CEECIND, FCT, Portugal
2016 | Marie Sklodowska-Curie Individual Fellowship, ref: 701730, European Commission
2008 | PhD Fellowship, ref: SFRH/BD/43406/2008, FCT, Portugal

Prémios:
2015 | Travel Award, EMBL Advanced Training Centre Partnership Programme Fellowship
2014 | InnoCentive Award: Real Time Monitoring of Steroid Metabolites and Metabolic Flux
2008 | BSc top3% merit prize, University of Coimbra, Portugal
2007 | BSc top3% merit prize, University of Coimbra, Portugal
2006 | BSc Student merit prize, BPI bank, Portugal

ORCID: 0000-0001-8176-4237
Researcher ID: AAD 9324 2021 
Scholar profile: https://scholar.google.com/citations?user=u_5gOV4AAAAJ&hl=en  
ResearchGate profile: https://www.researchgate.net/profile/Paulo-Gameiro